Alternative Test Methods Table

Conventional test method	Alternative Test Method	Infor- mation Last Updated
Skin Absorption
Skin Absorption: In Vivo Method (OECD TG 427)	Skin Absorption: In Vitro Method Description and references	OECD: Endorsed as equivalent to validated (2002)	OECD: TG 428 EU: Regulatory accepted as dermal percutaneous absorption test for the REACH program in place of in vivo animal studies (2008)	Replacement (When human skin is used) OR Reduction and Refinement (When skin from humanely killed animals is used)	April 2009

Conventional test method

Alternative Test Method

Infor- mation Last Updated

Name & Description

Validation Status

Regulatory Status

Effect or Potential Effect on Animal Use

Skin Absorption

Skin Absorption: In Vivo Method (OECD TG 427)

Skin Absorption: In Vitro Method
Description and references

OECD: Endorsed as equivalent to validated (2002)

OECD: TG 428

EU: Regulatory accepted as dermal percutaneous absorption test for the REACH program in place of in vivo animal studies (2008)

Replacement
(When human skin is used)

Reduction and Refinement
(When skin from humanely killed animals is used)

April 2009

List of Abbreviations