Alternative Test Methods Table

Conventional test method	Alternative Test Method				Infor- mation Last Updated
Conventional test method	Name & Description	Validation Status	Regulatory Status	Effect or Potential Effect on Animal Use	Infor- mation Last Updated
Vaccine Safety - Human Use
Abnormal Toxicity Test for Human Use Vaccines	Deletion of test Description and references	No information	EU: Accepted on condition of demonstrated consistency	Replacement	April 2009
Oral Polio Neurovirulence Test (Monkey intracerebral)	Mutant Analysis by PCR and Restriction Enzyme Cleavage, (MAPREC test) Description and references	WHO: Validated for type 3 oral polio virus vaccines	No information	Reduction	April 2009
Oral Polio Neurovirulence Test (Monkey intracerebral)	TgPVR21 Mouse Neurovirulence Test Description and references	WHO: Validated for types 1, 2 and 3 oral polio virus vaccines (2003)	No information	Replacement	March 2011
Residual Toxicity in Diptheria Vaccine (Guinea pig)	Vero Cell Test of Diphtheria Toxoid Vaccines Description and references	No information	EU: Accepted into PhEur	Replacement	April 2009